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This study aims to evaluate the epidemiology of burns in Dhaka. A survey form was employed to standardise the interview process. Survey form was prepared in English. Doctors were approached randomly at the local Burns unit at Dhaka, while they were at work. The two most common burn injuries were thermal 15 and electrical burns Other types of burns reported were chemical 9 , friction 7 , radiation 2 , lightning 1 and abuse 1. According to this group of local doctors, the complication of Burn Injuries which patients complain the most of was disfigurement. The high prevalence of burns, undeniably is attributed to anthropogenic reasons.

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Thus, burns are preventable, excluding that caused by natural disasters for example in this study, lightning. The consensus among these Dhaka doctors is that much effort is still needed to help the burns community. While the Dhaka authorities formulate their policies and plan their city, the authors recognise that a new tertiary level hospital is on the way in Dhaka. Bloodstream infection BSI is the dominant cause of death in severely burned patients.

With increasing antibiotic resistance, more attentions should be paid. The study subjects were all the severely burned patients diagnosed with BSI, whose blood samples was taken and cultured at least once.

The antibiotic susceptibility tests were performed. The patients with extreme more severe burns had significantly more kinds of bloodstream infection. Gram-negative bacilli were the main causative agents of BSI in severely burned patients Of all the pathogens, the top three species in terms of the detection rate were A.

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In conclusion, we carried out an etiological characterization of the severely burned patients with bloodstream infections admitted into BICU. Gram-negative MDR A. The etiologic agents of BSIs in our BICU differed from those previously reported studies suggesting a need in routine bacteria monitoring. It is also an important leading opportunistic pathogen, causing nosocomial infection. Gene interference is a necessary strategy to investigate the essential genes in P. To set up a gene interference platform in P. When an essential P. Invasive fungal infection is a challenge, a burden for the health sector due to high costs and high mortality rates.

Severe burn patients treated in ICU departments are in the group at high risk of invasive fungal infection. We diagnosed invasive fungal infections in severe burn patients based on evidence of microorganisms and monitor assessments of clinical and subclinical symptoms of patients. Total burn surface area was In the study, we found 2 species of fungal pathogens: Candida and Aspergillus.

Severe burn patients are at risk of invasive fungal infection from the second week after admission. Clinical symptoms are not specific but suggestive of diagnosis and correspond to subclinical microbiological results. Diabetic foot ulcers DFUs are one of the major complications in diabetes patients and can result in a high rate of amputation and mortality Its molecular pathology remains barely understood, impeding the development of effective treatments. Utilizing David Bioinformatics software, the selected differential genes were utilized to annotate the GO terms and pathways.

A total of 29 overlapped unregulated genes and 37 down regulated genes were identified. The majority of the DEGs were focused on apoptosis and the process of positive regulation of apoptosis.

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In the course of biology, the highest enrichment is in the process of cell cycle change. This can gain a better understanding of the molecular mechanisms and provide potentially valuable genes for prevention, diagnosis and treatment therapy. Multidrug-resistant MDR Gram-negative bacterial infections of skin wounds have been a major challenge in the treatment of burns and trauma.

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As one of the alternative strategies, PTT has recently become a research hotspot. However, the greatest defect for PTT could be the difficulty of targeting for the pathogenic bacteria to avoid the undesirable damage of ambient tissue. Furthermore, monotherapy may also be inefficient. To address these challenges, a novel graphene based nanocomposite with efficient dual-targeting ability for chemo-photothermal synergistic therapy was synthesized.

Aminobenzeneboronic acid B was grafted onto carboxyl graphene CG by the condensation reaction of amine groups on B and carboxyl groups on CG.

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The in vitro and in vivo toxicity was evaluated on the 3T3 fibroblast and mice, respectively. The MDR A. The targeting abilities for bacteria and biofilm were explored by the methods of SEM observation, fluorescent staining and thermal imaging. The in vitro antibacterial and anti-biofilm efficiencies were determined using the standard plate count method. The in vivo antibacterial and anti-biofilm efficiencies were investigated in the murine skin wound infection model and subcutaneous abscess model, respectively.

In vitro , B-G-QAS mediated chemo-photothermal therapy can effectively kill the MDR Gram-negative bacteria and biofilms; in vivo, it can also ablate the bacterial and biofilm infection, significantly accelerating wound healing. B-G-QAS shows excellent biocompatibility in vitro and in vivo. Invasive refractory infection is one of the common complications of wounds by various pathogens. This kind wound easily induces systemic infection, even sepsis.

The most important treatment for the invasive refractory infectious wound is to remove the local pathogens effectively and immediately. It is difficult to delete the pathogens and devitalized tissues precisely by the conventional methods. Hydrodynamic debridement was reported suitable to treat different wounds for its accuracy and convenience. In this study we intend to observe the clinical effects of hydrodynamic debridement on refractory infectious wounds. From to , there were 32 cases with refractory infectious wounds involved in this study, in which 28 cases were induced by burn injury.

All the wounds were treated with a hydrodynamic debridement system versajet-II. The clinical effects were observed and recorded after the treatment. After the hydrodynamic debridement, 32 patients were immediately underwent skin grafting or suture directly. Moreover, the induced systemic infections were effectively controlled. There was no patient died in this study. The hydrodynamic debridement system is one of the effective clinical methods to treat refractory infectious wounds.

The effect of pulmonary protective ventilation strategy combined with lung recruitment on hemodynamics in patients with severe burns complicated with ARDS with propofol, midazolam and dexmedetomidine sedation. Thirty patients with severe burns complicated with ARDS were randomly divided into three groups. They were sedated with propofol, midazolam and dexmedetomidine. Mechanical ventilation was also performed in the pulmonary protective ventilation strategy.

The treatment status and results of the patients were counted. Repeated measures analysis of variance and LSD test for data row single data. The emergence of carbapenem resistant Klebsieila pneumoniae CRKP is an important threat to burned patients. This retrospective study was conducted to collect information from burned patients in Shanghai Ruijin Hospital between and A significant increase in number of CRKP isolated from burned patients was observed between and CRKP were resistant to almost all available antimicrobials and are susceptible only to polymyxins, tigecycline.

A minority of CRKP are also susceptible to the few remaining aminoglycosides. Antibiotics which can been currently used to treat CRKP infections include polymyxins, tigecycline and some type of aminoglycosides.

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The efficacy of combination antibiotics therapy is better than single-agent therapy for the treatment of CRKP bloodstream infections. Acinetobacter baumannii A. In this study, we developed a new 15ul reaction system of recombinase polymerase amplification RPA for rapid detection of A.

We collected 30 A. We evaluated the sensitivity, specificity and the detection rate of clinical isolates. The 15ul-RPA detected successfully A. The specificity of two identification genes was also extremely high, no positive amplification signal in non- Acinetobacter was observed. The detection rate of A. Furthermore, we tested the bla OXA resistance gene of A. Compared with the results of the antibiotic susceptibility test, we found that all carbapenem-resistant A.

We consider that 15ul-RPA has potential as promising alternative molecular diagnostic method to rapid and effective detection of A. The quantitative cultures are more valuable in prediction of metastatic invasion of organisms. The effect of conventional silver sulphadiazine dressing with colloidal nanosilver hydrogel dressing in burn wounds needs to be compared based on quatitative cultures.


Wound cultures for qualitative and quantitative analyses were taken at the end of first week, second , third and fourth week. In Nanocrystalline Silver group average bacterial load of Klebsiella progressively decreased Average bacterial load of E. There was progressive increase in average bacterial load of Pseudomonas during second and third weeks.

The increase continued in the fourth week.